LBMC » NucleoMiner

Arabic to Roman pair list.

--------------------------

Description

~~~~~~~~~~~

Utility to convert Arabic numbers to Roman numbers

Usage

~~~~~

::

    ARAB2ROM()

Author(s)

~~~~~~~~~

Florent Chuffart

R: False Discovery Rate

False Discovery Rate

--------------------

Description

~~~~~~~~~~~

From a vector x of independent p-values, extract the cutoff

corresponding to the specified FDR. See Benjamini & Hochberg 1995 paper

Usage

~~~~~

::

    FDR(x, FDR)

Arguments

~~~~~~~~~

``x``

A vector x of independent p-values.

``FDR``

The specified FDR.

Value

~~~~~

Return the the corresponding cutoff.

Author(s)

~~~~~~~~~

Gael Yvert, Florent Chuffart

Examples

~~~~~~~~

::

    print("example")

R: Roman to Arabic pair list.

Roman to Arabic pair list.

--------------------------

Description

~~~~~~~~~~~

Utility to convert Roman numbers into Arabic numbers

Usage

~~~~~

::

    ROM2ARAB()

Author(s)

~~~~~~~~~

Florent Chuffart

R: Aggregate replicated sample's nucleosomes.

Aggregate replicated sample's nucleosomes.

------------------------------------------

Description

~~~~~~~~~~~

This function aggregates nucleosomes from replicated samples. It uses

TemplateFilter ouput of each sample as replicate. Each sample owns a set

of nucleosomes computed using TemplateFilter and ordered by the position

of their center (dyad). A chain of nucleosomes is builts across all

replicates. Adjacent nucleosomes of the chain are compared two by two.

Comparison is based on a log likelihood ratio (LLR1). depending on the

LLR1 value nucleosomes are merged (low LLR) or separated (high LLR).

Finally the function returns a list of clusters and all computed

llr\_scores. Each cluster ows an attribute wp for "well positioned".

This attribute is set to TRUE if the cluster is composed of exactly one

nucleosome of each sample.

Usage

~~~~~

::

    aggregate_intra_strain_nucs(samples, llr_thres = 20, coord_max = 2e+07)

Arguments

~~~~~~~~~

``samples``

A list of samples. Each sample is a list like *sample = list(id=...,

marker=..., strain=..., roi=..., inputs=..., outputs=...)* with *roi =

list(name=..., begin=..., end=..., chr=..., genome=...)*.

``llr_thres``

Log likelihood ratio threshold to decide between merging and separating

``coord_max``

A too big value to be a coord for a nucleosome lower bound.

Value

~~~~~

Returns a list of clusterized nucleosomes, and all computed llr scores.

Author(s)

~~~~~~~~~

Florent Chuffart

Examples

~~~~~~~~

::

    # Dealing with a region of interest

    roi =list(name="example", begin=1000,  end=1300, chr="1", genome=rep("A",301))

    samples = list()

    for (i in 1:3) {

        # Create TF output

        tf_nuc = list("chr"=paste("chr", roi$chr, sep=""), "center"=(roi$end + roi$begin)/2, "width"= 150, "correlation.score"= 0.9)

        outputs = dfadd(NULL,tf_nuc)

        outputs = filter_tf_outputs(outputs, roi$chr, roi$begin, roi$end)

        # Generate corresponding reads

        nb_reads = round(runif(1,170,230))

        reads = round(rnorm(nb_reads, tf_nuc$center,20))

        u_reads = sort(unique(reads))

        strands = sample(c(rep("R",ceiling(length(u_reads)/2)),rep("F",floor(length(u_reads)/2))))

        counts = apply(t(u_reads), 2, function(r) { sum(reads == r)})

        shifts = apply(t(strands), 2, function(s) { if (s == "F") return(-tf_nuc$width/2) else return(tf_nuc$width/2)})

        u_reads = u_reads + shifts

        inputs = data.frame(list("V1" = rep(roi$chr, length(u_reads)),

                                 "V2" = u_reads,

                                                         "V3" = strands,

                                                         "V4" = counts), stringsAsFactors=FALSE)

        samples[[length(samples) + 1]] = list(id=1, marker="Mnase_Seq", strain="strain_ex", total_reads = 10000000, roi=roi, inputs=inputs, outputs=outputs)

    }

    print(aggregate_intra_strain_nucs(samples))

R: Aligns nucleosomes between 2 strains.

Aligns nucleosomes between 2 strains.

-------------------------------------

Description

~~~~~~~~~~~

This function aligns nucleosomes between two strains for a given genome

region.

Usage

~~~~~

::

    align_inter_strain_nucs(replicates, wp_nucs_strain_ref1 = NULL, 

        wp_nucs_strain_ref2 = NULL, corr_thres = 0.5, llr_thres = 100, 

        config = NULL, ...)

Arguments

~~~~~~~~~

``replicates``

Set of replicates, ideally 3 per strain.

``wp_nucs_strain_ref1``

List of aggregates nucleosome for strain 1. If it's NULL this list will

be computed.

``wp_nucs_strain_ref2``

List of aggregates nucleosome for strain 2. If it's NULL this list will

be computed.

``corr_thres``

Correlation threshold.

``llr_thres``

Log likelihood ratio threshold to decide between merging and separating

``config``

GLOBAL config variable

``...``

A list of parameters that will be passed to

*aggregate\_intra\_strain\_nucs* if needed.

Value

~~~~~

Returns a list of clusterized nucleosomes, and all computed llr scores.

Author(s)

~~~~~~~~~

Florent Chuffart

Examples

~~~~~~~~

::

        # Define new translate_cur function...

        translate_cur = function(roi, strain2, big_cur=NULL, config=NULL) {

          return(roi)

        }

        # Binding it by uncomment follwing lines.

        unlockBinding("translate_cur", as.environment("package:nucleominer"))

        unlockBinding("translate_cur", getNamespace("nucleominer"))

        assign("translate_cur", translate_cur, "package:nucleominer")

        assign("translate_cur", translate_cur, getNamespace("nucleominer"))

        lockBinding("translate_cur", getNamespace("nucleominer"))

        lockBinding("translate_cur", as.environment("package:nucleominer"))

    # Dealing with a region of interest

    roi =list(name="example", begin=1000,  end=1300, chr="1", genome=rep("A",301), strain_ref1 = "STRAINREF1")

    roi2 = translate_cur(roi, roi$strain_ref1)

    replicates = list()

    for (j in 1:2) {

        samples = list()

        for (i in 1:3) {

            # Create TF output

            tf_nuc = list("chr"=paste("chr", roi$chr, sep=""), "center"=(roi$end + roi$begin)/2, "width"= 150, "correlation.score"= 0.9)

            outputs = dfadd(NULL,tf_nuc)

            outputs = filter_tf_outputs(outputs, roi$chr, roi$begin, roi$end)

            # Generate corresponding reads

            nb_reads = round(runif(1,170,230))

            reads = round(rnorm(nb_reads, tf_nuc$center,20))

            u_reads = sort(unique(reads))

            strands = sample(c(rep("R",ceiling(length(u_reads)/2)),rep("F",floor(length(u_reads)/2))))

            counts = apply(t(u_reads), 2, function(r) { sum(reads == r)})

            shifts = apply(t(strands), 2, function(s) { if (s == "F") return(-tf_nuc$width/2) else return(tf_nuc$width/2)})

            u_reads = u_reads + shifts

            inputs = data.frame(list("V1" = rep(roi$chr, length(u_reads)),

                                     "V2" = u_reads,

                                                             "V3" = strands,

                                                             "V4" = counts), stringsAsFactors=FALSE)

            samples[[length(samples) + 1]] = list(id=1, marker="Mnase_Seq", strain=paste("strain_ex",j,sep=""), total_reads = 10000000, roi=roi, inputs=inputs, outputs=outputs)

        }

        replicates[[length(replicates) + 1]] = samples

    }

    print(align_inter_strain_nucs(replicates))

R: Launch DESeq methods.

Launch DESeq methods.

---------------------

Description

~~~~~~~~~~~

This function is based on DESeq example. It normalizes data, fit data to

GLM model with and without interaction term and compares the two models.

Usage

~~~~~

::

    analyse_design(snep_design, reads)

Arguments

~~~~~~~~~

``snep_design``

The design to consider.

``reads``

The data to consider.

Author(s)

~~~~~~~~~

Florent Chuffart

R: Stage replicates data

Stage replicates data

---------------------

Description

~~~~~~~~~~~

This function loads in memory the data corresponding to the given

experiments.

Usage

~~~~~

::

    build_replicates(expe, roi, only_fetch = FALSE, get_genome = FALSE, 

        all_samples, config = NULL)

Arguments

~~~~~~~~~

``expe``

a list of vectors corresponding to replicates.

``roi``

the region that we are interested in.

``only_fetch``

filter or not inputs.

``get_genome``

Load or not corresponding genome.

``all_samples``

Global list of samples.

``config``

GLOBAL config variable.

Author(s)

~~~~~~~~~

Florent Chuffart

Examples

~~~~~~~~

::

    # library(rjson)

    # library(nucleominer)

    #

    # # Read config file

    # json_conf_file = "nucleominer_config.json"

    # config = fromJSON(paste(readLines(json_conf_file), collapse=""))

    # # Read sample file

    # all_samples = get_content(config$CSV_SAMPLE_FILE, "cvs", sep=";", head=TRUE, stringsAsFactors=FALSE)

    # # here are the sample ids in a list

    # expes = list(c(1))

    # # here is the region that we wnt to see the coverage

    # cur = list(chr="8", begin=472000, end=474000, strain_ref="BY")

    # # it displays the corverage

    # replicates = build_replicates(expes, cur, all_samples=all_samples, config=config)

    # out = watch_samples(replicates, config$READ_LENGTH,

    #       plot_coverage = TRUE,

    #       plot_squared_reads = FALSE,

    #       plot_ref_genome = FALSE,

    #       plot_arrow_raw_reads = FALSE,

    #       plot_arrow_nuc_reads = FALSE,

    #       plot_gaussian_reads = FALSE,

    #       plot_gaussian_unified_reads = FALSE,

    #       plot_ellipse_nucs = FALSE,

    #       plot_wp_nucs = FALSE,

    #       plot_wp_nuc_model = FALSE,

    #       plot_common_nucs = FALSE,

    #       height = 50)

R: Extract a sub part of the corresponding c2c file

Extract a sub part of the corresponding c2c file

------------------------------------------------

Description

~~~~~~~~~~~

This fonction allows to access to a specific part of the c2c file.

Usage

~~~~~

::

    c2c_extraction(strain1, strain2, chr = NULL, lower_bound = NULL, 

        upper_bound = NULL, config = NULL)

Arguments

~~~~~~~~~

``strain1``

the key strain

``strain2``

the target strain

``chr``

if defined, the c2c will be filtered according to the chromosome value

``lower_bound``

if defined, the c2c will be filtered for part of the genome upper than

lower\_bound

``upper_bound``

if defined, the c2c will be filtered for part of the genome lower than

upper\_bound

``config``

GLOBAL config variable

Author(s)

~~~~~~~~~

Florent Chuffart

R: reformat an "apply manipulated" list of regions

reformat an "apply manipulated" list of regions

-----------------------------------------------

Description

~~~~~~~~~~~

Utils to reformat an "apply manipulated" list of regions

Usage

~~~~~

::

    collapse_regions(regions)

Arguments

~~~~~~~~~

+---------------+----+

| ``regions``   |    |

+---------------+----+

Author(s)

~~~~~~~~~

Florent Chuffart

R: Compute Common Uninterrupted Regions (CUR)

Compute Common Uninterrupted Regions (CUR)

------------------------------------------

Description

~~~~~~~~~~~

CURs are regions that can be aligned between the genomes

Usage

~~~~~

::

    compute_inter_all_strain_curs(diff_allowed = 30, min_cur_width = 4000, 

        config = NULL)

Arguments

~~~~~~~~~

``diff_allowed``

the maximum indel width allowe din a CUR

``min_cur_width``

The minimum width of a CUR

``config``

GLOBAL config variable

Author(s)

~~~~~~~~~

Florent Chuffart

R: Crop bound of regions according to region of interest bound

Crop bound of regions according to region of interest bound

-----------------------------------------------------------

Description

~~~~~~~~~~~

The fucntion is no more necessary since we remove "big\_cur" bug in

translate\_cur function.

Usage

~~~~~

::

    crop_fuzzy(tmp_fuzzy_nucs, roi, strain, config = NULL)

Arguments

~~~~~~~~~

``tmp_fuzzy_nucs``

the regiuons to be croped.

``roi``

The region of interest.

``strain``

The strain to consider.

``config``

GLOBAL config variable

Author(s)

~~~~~~~~~

Florent Chuffart

R: Adding list to a dataframe.

Adding list to a dataframe.

---------------------------

Description

~~~~~~~~~~~

Add a list *l* to a dataframe *df*. Create it if *df* is *NULL*. Return

the dataframe *df*.

Usage

~~~~~

::

    dfadd(df, l)

Arguments

~~~~~~~~~

``df``

A dataframe

``l``

A list

Value

~~~~~

Return the dataframe *df*.

Author(s)

~~~~~~~~~

Florent Chuffart

Examples

~~~~~~~~

::

    ## Here dataframe is NULL

    print(df)

    df = NULL

    # Initialize df

    df = dfadd(df, list(key1 = "value1", key2 = "value2"))

    print(df)

    # Adding elements to df

    df = dfadd(df, list(key1 = "value1'", key2 = "value2'"))

    print(df)

R: Prefetch data

Prefetch data

-------------

Description

~~~~~~~~~~~

Fetch and filter inputs and outpouts per region of interest. Organize it

per replicates.

Usage

~~~~~

::

    fetch_mnase_replicates(strain, roi, all_samples, config = NULL, 

        only_fetch = FALSE, get_genome = FALSE, get_ouputs = TRUE)

Arguments

~~~~~~~~~

``strain``

The strain we want mnase replicatesList of replicates. Each replicates

is a vector of sample ids.

``roi``

Region of interest.

``all_samples``

Global list of samples.

``config``

GLOBAL config variable

``only_fetch``

If TRUE, only fetch and not filtering. It is used tio load sample files

into memory before forking.

``get_genome``

If TRUE, load corresponding genome sequence.

``get_ouputs``

If TRUE, get also ouput corresponding TF output files.

Author(s)

~~~~~~~~~

Florent Chuffart

R: Filter TemplateFilter inputs

Filter TemplateFilter inputs

----------------------------

Description

~~~~~~~~~~~

This function filters TemplateFilter inputs according genome area

observed properties. It takes into account reads that are at the

frontier of this area and the strand of these reads.

Usage

~~~~~

::

    filter_tf_inputs(inputs, chr, x_min, x_max, nuc_width = 160, 

        only_f = FALSE, only_r = FALSE, filter_for_coverage = FALSE, 

        USE_DPLYR = TRUE)

Arguments

~~~~~~~~~

``inputs``

TF inputs to be filtered.

``chr``

Chromosome observed, here chr is an integer.

``x_min``

Coordinate of the first bp observed.

``x_max``

Coordinate of the last bp observed.

``nuc_width``

Nucleosome width.

``only_f``

Filter only F reads.

``only_r``

Filter only R reads.

``filter_for_coverage``

Does it filter for plot coverage?

``USE_DPLYR``

Use dplyr lib to filter reads.

Value

~~~~~

Returns filtred inputs.

Author(s)

~~~~~~~~~

Florent Chuffart

R: Filter TemplateFilter outputs

Filter TemplateFilter outputs

-----------------------------

Description

~~~~~~~~~~~

This function filters TemplateFilter outputs according, not only genome

area observerved properties, but also correlation and overlapping

threshold.

Usage

~~~~~

::

    filter_tf_outputs(tf_outputs, chr, x_min, x_max, nuc_width = 160, 

        ol_bp = 59, corr_thres = 0.5)

Arguments

~~~~~~~~~

``tf_outputs``

TemplateFilter outputs.

``chr``

Chromosome observed, here chr is an integer.

``x_min``

Coordinate of the first bp observed.

``x_max``

Coordinate of the last bp observed.

``nuc_width``

Nucleosome width.

``ol_bp``

Overlap Threshold.

``corr_thres``

Correlation threshold.

Value

~~~~~

Returns filtered TemplateFilter Outputs

Author(s)

~~~~~~~~~

Florent Chuffart

R: to flat aggregate\_intra\_strain\_nucs function output

to flat aggregate\_intra\_strain\_nucs function output

------------------------------------------------------

Description

~~~~~~~~~~~

This function builds a dataframe of all clusters obtain from

aggregate\_intra\_strain\_nucs function.

Usage

~~~~~

::

    flat_aggregated_intra_strain_nucs(partial_strain_maps, cur_index, 

        nb_tracks = 3)

Arguments

~~~~~~~~~

``partial_strain_maps``

the output of aggregate\_intra\_strain\_nucs function

``cur_index``

the index of the roi involved

``nb_tracks``

the number of replicates

Value

~~~~~

Returns a dataframe of all clusters obtain from

aggregate\_intra\_strain\_nucs function.

Author(s)

~~~~~~~~~

Florent Chuffart

R: flat reads

flat reads

----------

Description

~~~~~~~~~~~

Extract reads coordinates from TempleteFilter input sequence

Usage

~~~~~

::

    flat_reads(reads, nuc_width)

Arguments

~~~~~~~~~

``reads``

TemplateFilter input reads

``nuc_width``

Width used to shift F and R reads.

Value

~~~~~

Returns a list of F reads, R reads and joint/shifted F and R reads.

Author(s)

~~~~~~~~~

Florent Chuffart

R: Retrieve Reads

Retrieve Reads

--------------

Description

~~~~~~~~~~~

Retrieve reads for a given marker, combi, form.

Usage

~~~~~

::

    get_all_reads(marker, combi, form = "wp", config = NULL)

Arguments

~~~~~~~~~

``marker``

The marker to considere.

``combi``

The starin combination to considere.

``form``

The nuc form to considere.

``config``

GLOBAL config variable

Author(s)

~~~~~~~~~

Florent Chuffart

R: get comp strand

get comp strand

---------------

Description

~~~~~~~~~~~

Compute the complementatry strand.

Usage

~~~~~

::

    get_comp_strand(strand)

Arguments

~~~~~~~~~

``strand``

The original strand.

Value

~~~~~

Returns the complementatry strand.

Author(s)

~~~~~~~~~

Florent Chuffart

R: Build the design for DESeq

Build the design for DESeq

--------------------------

Description

~~~~~~~~~~~

This function build the design according sample properties.

Usage

~~~~~

::

    get_design(marker, combi, all_samples)

Arguments

~~~~~~~~~

``marker``

The marker to considere.

``combi``

The starin combination to considere.

``all_samples``

Global list of samples.

Author(s)

~~~~~~~~~

Florent Chuffart

R: Compute the fuzzy list for a given strain.

Compute the fuzzy list for a given strain.

------------------------------------------

Description

~~~~~~~~~~~

This function grabs the nucleosomes detxted by template\_filter that

have been rejected bt aggregate\_intra\_strain\_nucs as well positions.

Usage

~~~~~

::

    get_intra_strain_fuzzy(wp_map, roi, strain, config = NULL)

Arguments

~~~~~~~~~

``wp_map``

Well positionned nucleosomes map.

``roi``

The region of interest.

``strain``

The strain we want to extracvt the fuzzy map.

``config``

GLOBAL config variable.

Author(s)

~~~~~~~~~

Florent Chuffart

R: Compute the list of SNEPs for a given set of marker, strain...

Compute the list of SNEPs for a given set of marker, strain combination and nuc form.

-------------------------------------------------------------------------------------

Description

~~~~~~~~~~~

This function uses

Usage

~~~~~

::

    get_sneps(marker, combi, form, all_samples, FDR = 1e-04, config = NULL)

Arguments

~~~~~~~~~

``marker``

The marker involved.

``combi``

The strain combination involved.

``form``

the nuc form involved.

``all_samples``

Global list of samples.

``FDR``

``config``

GLOBAL config variable

Author(s)

~~~~~~~~~

Florent Chuffart

Examples

~~~~~~~~

::

    marker = "H3K4me1"

    combi = c("BY", "YJM")

    form = "wpunr" # "wp" | "unr" | "wpunr"

    # foo = get_sneps(marker, combi, form)

    # foo = get_sneps("H4K12ac", c("BY", "RM"), "wp")

R: Compute the unaligned nucleosomal regions (UNRs).

Compute the unaligned nucleosomal regions (UNRs).

-------------------------------------------------

Description

~~~~~~~~~~~

This function aggregate non common wp nucs for each strain and substract

common wp nucs. It does not take care about the size of the resulting

UNR. It will be take into account in the count read part og the

pipeline.

Usage

~~~~~

::

    get_unrs(combi, roi, cur_index, wp_maps, fuzzy_maps, common_nuc_results, 

        config = NULL)

Arguments

~~~~~~~~~

``combi``

The strain combination to consider.

``roi``

The region of interest.

``cur_index``

The region of interest index.

``wp_maps``

Well positionned nucleosomes maps.

``fuzzy_maps``

Fuzzy nucleosomes maps.

``common_nuc_results``

Common wp nuc maps

``config``

GLOBAL config variable

Author(s)

~~~~~~~~~

Florent Chuffart

R: Returns the intersection of 2 list on regions.

Returns the intersection of 2 list on regions.

----------------------------------------------

Description

~~~~~~~~~~~

This function...

Usage

~~~~~

::

    intersect_region(region1, region2)

Arguments

~~~~~~~~~

``region1``

Original regions.

``region2``

Regions to intersect.

Author(s)

~~~~~~~~~

Florent Chuffart

R: Likelihood ratio

Likelihood ratio

----------------

Description

~~~~~~~~~~~

Compute the log likelihood ratio of two or more set of value.

Usage

~~~~~

::

    llr_score_nvecs(xs)

Arguments

~~~~~~~~~

``xs``

list of vectors.

Value

~~~~~

Returns the log likelihood ratio.

Author(s)

~~~~~~~~~

Florent Chuffart

Examples

~~~~~~~~

::

    # LLR score for 2 set of values

    mean1=5; sd1=2; card2 = 250

    mean2=6; sd2=3; card1 = 200

    x1 = rnorm(card1, mean1, sd1)

    x2 = rnorm(card2, mean2, sd2)

    min = floor(min(c(x1,x2)))

    max = ceiling(max(c(x1,x2)))

    hist(c(x1,x2), xlim=c(min, max), breaks=min:max)

    lines(min:max,dnorm(min:max,mean1,sd1)*card1,col=2)

    lines(min:max,dnorm(min:max,mean2,sd2)*card2,col=3)

    lines(min:max,dnorm(min:max,mean(c(x1,x2)),sd(c(x1,x2)))*card2,col=4)

    llr_score_nvecs(list(x1,x2))

R: nm

nm

--

Description

~~~~~~~~~~~

It provides a set of useful functions allowing to perform quantitative

analysis of nucleosomal epigenome.

Details

~~~~~~~

+---------------+---------------------------------------------------+

| Package:      | nucleominer                                       |

+---------------+---------------------------------------------------+

| Maintainer:   | Florent Chuffart <florent.chuffart@ens-lyon.fr>   |

+---------------+---------------------------------------------------+

| Author:       | Florent Chuffart                                  |

+---------------+---------------------------------------------------+

| Version:      | 2.3.47                                            |

+---------------+---------------------------------------------------+

| License:      | CeCILL                                            |

+---------------+---------------------------------------------------+

| Title:        | nm                                                |

+---------------+---------------------------------------------------+

| Depends:      | seqinr, plotrix, DESeq, cachecache, dplyr         |

+---------------+---------------------------------------------------+

Author(s)

~~~~~~~~~

Florent Chuffart

R: Plot the distribution of reads.

Plot the distribution of reads.

-------------------------------

Description

~~~~~~~~~~~

This fuxntion use the DESeq nomalization feature to compare

qualitatively the distribution.

Usage

~~~~~

::

    plot_dist_samples(strain, marker, res, all_samples, NEWPLOT = TRUE)

Arguments

~~~~~~~~~

``strain``

The strain to considere.

``marker``

The marker to considere.

``res``

Data

``all_samples``

Global list of samples.

``NEWPLOT``

If FALSE the curve will be add to the current plot.

Author(s)

~~~~~~~~~

Florent Chuffart

R: sign from strand

sign from strand

----------------

Description

~~~~~~~~~~~

Get the sign of strand

Usage

~~~~~

::

    sign_from_strand(strands)

Arguments

~~~~~~~~~

+---------------+----+

| ``strands``   |    |

+---------------+----+

Value

~~~~~

If strand in forward then returns 1 else returns -1

Author(s)

~~~~~~~~~

Florent Chuffart

R: Substract to a list of regions an other list of regions that...

Substract to a list of regions an other list of regions that intersect it.

--------------------------------------------------------------------------

Description

~~~~~~~~~~~

This fucntion embed a recursive part. It occurs when a substracted

region split an original region on two.

Usage

~~~~~

::

    substract_region(region1, region2)

Arguments

~~~~~~~~~

``region1``

Original regions.

``region2``

Regions to substract.

Author(s)

~~~~~~~~~

Florent Chuffart

R: Switch a pairlist

Switch a pairlist

-----------------

Description

~~~~~~~~~~~

Take a pairlist key:value and return the switched pairlist value:key.

Usage

~~~~~

::

    switch_pairlist(l)

Arguments

~~~~~~~~~

``l``

The pairlist to switch.

Value

~~~~~

The switched pairlist.

Author(s)

~~~~~~~~~

Florent Chuffart

Examples

~~~~~~~~

::

    l = list(key1 = "value1", key2 = "value2")

    print(switch_pairlist(l))

R: Translate coords of a genome region.

Translate coords of a genome region.

------------------------------------

Description

~~~~~~~~~~~

This function is used in the examples, usualy you have to define your

own translation function and overwrite this one using *unlockBinding*

features. Please, refer to the example.

Usage

~~~~~

::

    translate_cur(roi, strain2, config = NULL, big_cur = NULL)

Arguments

~~~~~~~~~

``roi``

Original genome region of interest.

``strain2``

The strain in wich you want the genome region of interest.

``config``

GLOBAL config variable

``big_cur``

A largest region than roi use to filter c2c if it is needed.

Author(s)

~~~~~~~~~

Florent Chuffart

Examples

~~~~~~~~

::

    # Define new translate_cur function...

    translate_cur = function(roi, strain2, config) {

        strain1 = roi$strain_ref

        if (strain1 == strain2) {

            return(roi)

        } else {

          stop("Here is my new translate_cur function...")

        }

    }

    # Binding it by uncomment follwing lines.

    # unlockBinding("translate_cur", as.environment("package:nm"))

    # unlockBinding("translate_cur", getNamespace("nm"))

    # assign("translate_cur", translate_cur, "package:nm")

    # assign("translate_cur", translate_cur, getNamespace("nm"))

    # lockBinding("translate_cur", getNamespace("nm"))

    # lockBinding("translate_cur", as.environment("package:nm"))

R: Translate a list of regions from a strain ref to another.

Translate a list of regions from a strain ref to another.

---------------------------------------------------------

Description

~~~~~~~~~~~

This function is an elaborated call to translate\_cur.

Usage

~~~~~

::

    translate_regions(regions, combi, cur_index, config = NULL, roi)

Arguments

~~~~~~~~~

``regions``

Regions to be translated.

``combi``

Combination of strains.

``cur_index``

The region of interest index.

``config``

GLOBAL config variable

``roi``

The region of interest.

Author(s)

~~~~~~~~~

Florent Chuffart

R: Aggregate regions that intersect themselves.

Aggregate regions that intersect themselves.

--------------------------------------------

Description

~~~~~~~~~~~

This function is based on sort of lower bounds to detect regions that

intersect. We compare lower bound and upper bound of the porevious item.

This function embed a while loop and break break regions list become

stable.

Usage

~~~~~

::

    union_regions(regions)

Arguments

~~~~~~~~~

``regions``

The Regions to be aggregated

Author(s)

~~~~~~~~~

Florent Chuffart

R: Watching analysis of samples

Watching analysis of samples

----------------------------

Description

~~~~~~~~~~~

This function allows to view analysis for a particuler region of the

genome.

Usage

~~~~~