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Readme / Documentation for *NucleoMiner2*
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*****************************************
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*NucleoMiner2* offers Python API and R package allowing to perform
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quantitative analysis of nucleosomal epigenome. It is especially well
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suited for scripting to extract natural Single-Nucleosome Epi-
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Polymorphisms (SNEP) from ChIP-Seq data.
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License
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=======
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Copyright CNRS 2012-2013
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* Florent CHUFFART
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* Jean-Baptiste VEYRIERAS
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* Gael YVERT
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This software is a computer program which purpose is to perform
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quanti- tative analysis of epigenetic marks at single nucleosome
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resolution.
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This software is governed by the CeCILL license under French law and
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abiding by the rules of distribution of free software.  You can  use,
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modify and/ or redistribute the software under the terms of the CeCILL
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license as circulated by CEA, CNRS and INRIA at the following URL
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"http://www.cecill.info".
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As a counterpart to the access to the source code and  rights to copy,
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modify and redistribute granted by the license, users are provided
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only with a limited warranty  and the software's author,  the holder
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of the economic rights,  and the successive licensors  have only
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limited liability.
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In this respect, the user's attention is drawn to the risks associated
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with loading,  using,  modifying and/or developing or reproducing the
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software by the user in light of its specific status of free software,
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that may mean  that it is complicated to manipulate,  and  that  also
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therefore means  that it is reserved for developers  and  experienced
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professionals having in-depth computer knowledge. Users are therefore
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encouraged to load and test the software's suitability as regards
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their requirements in conditions enabling the security of their
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systems and/or data to be ensured and,  more generally, to use and
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operate it in the same conditions as regards security.
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The fact that you are presently reading this means that you have had
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knowledge of the CeCILL license and that you accept its terms.
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Installation Instructions
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=========================
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Links
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-----
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*NucleoMiner2* home page and documentation: https://forge.cbp.ens-
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lyon.fr/redmine/projects/nucleominer
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Gael Yvert lab page: http://www.ens-lyon.fr/LBMC/gisv/
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Installation
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------------
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   * Download archive
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   * Compile bowtie2
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   * Compile samtools
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   * Compile bedtools
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   * Compile TemplateFilter
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Required R pacakes:
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   * bot - fork
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   * rjson
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   * seqinr
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   cd src/r_packages/
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         tar xfvz R-latest.tar.gz
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         cd R-patched
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         ./configure --with-x=no PDFLATEX="ls"
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         make
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   cd ../../..
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   R_BIN=src/r_packages/R-patched/bin/R
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         $R_BIN CMD INSTALL src/r_packages/rjson_0.2.12.tar.gz
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         $R_BIN CMD INSTALL src/r_packages/seqinr_3.0-7.tar.gz
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         $R_BIN CMD INSTALL src/r_packages/plotrix_3.4-5.tar.gz
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         $R_BIN CMD INSTALL src/r_packages/nm_2.0.tar.gz
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         $R_BIN CMD INSTALL src/r_packages/fork_1.2.4.tar.gz
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         $R_BIN CMD INSTALL src/r_packages/bot_0.9.tar.gz
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         $R_BIN CMD INSTALL src/r_packages/DESeq_1.14.0.tar.gz
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...
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usage
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=====
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See html documentation for *NucleoMiner2*: http://www.ens-
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lyon.fr/LBMC/gisv/