Révision c25275e2 doc/sphinx_doc/tuto.rst
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APPENDICE: Generate .c2c Files |
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------------------------------ |
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$$$ TODO make it works properly. |
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working directory. |
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The `.c2c` files is a simple table that describes how the genome sequence can be aligned. We generate it using some NucleoMiner 1.0 scripts. |
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The `.c2c` files is a simple table that describes how the genome sequence can be aligned. We generate it using NucleoMiner 1.0.
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To use NucleoMiner 1.0 scripts on your UNIX/LINUX computer you need first to install MUMmer which is a system for rapidly aligning entire genomes, whether in complete or draft form.
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To install NucleoMiner 1.0 on your UNIX/LINUX computer you need first to install the Genetic Data analysis Library (GDL), which is a dynamic library of useful C functions derived from the GNU Scientific Library. |
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Installing the GDL library |
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^^^^^^^^^^^^^^^^^^^^^^^^^^ |
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Installing the MUMmer library |
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^^^^^^^^^^^^^^^^^^^^^^^^^^^^^ |
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Get the gdl-1.0.tar.gz archive on your computer (in the directory deps of your working directory). Copy it in a dedicated directory. Go into this directory using the cd command, and then unfold the archive by typing:
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Get the last version of MUMmer archive on your computer (MUMmer3.23.tar.gz distributed in the directory deps of your working directory). Copy it in a dedicated directory. Install it locally into the src folder of you working directory by typing (working directory):
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tar -xvzf gdl-1.0.tar.gz |
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.. code:: bash |
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mkdir tmp_c2c_workdir |
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cd tmp_c2c_workdir |
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cp ../deps/gdl-1.0.tar.gz . |
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tar -xvzf gdl-1.0.tar.gz |
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cd gdl-1.0 |
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./configure |
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make |
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cd .. |
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Now you need to install the library on your system. This needs root priviledges: |
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cd src |
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tar xfvz ../deps/MUMmer3.23.tar.gz |
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cd MUMmer3.23 |
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make check |
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make install |
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.. code:: bash |
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sudo make install |
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Installing NucleoMiner 1.0 |
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^^^^^^^^^^^^^^^^^^^^^^^^^^ |
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Installing NucleoMiner 1.0 scripts |
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^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^ |
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Get the nucleominer-1.0.tar.gz archive on your computer. Copy it in a dedicated directory. Go into this directory using the cd command, and then unfold the archive by typing:
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Get the nucleominer-1.0.tar.gz archive on your computer (distributed in the directory deps of your working directory). Install it locally into the src folder of you working directory by typing (working directory):
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This creates a directory called nucleominer-1.0. You now need to go into this directory and compile the library, by typing: |
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.. code:: bash |
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cp ../deps/nucleominer-1.0.tar.gz . |
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tar -xvzf nucleominer-1.0.tar.gz |
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cd nucleominer-1.0 |
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ln -s ../gdl-1.0/gdl |
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./configure |
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make |
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You can then use the binaries dircetly from this folder (best then is to add the path to this folder in your PATH environment variable). If you want to install nucleominer at the system's level (useful if mutiple users will need it) then type, with root priviledges: |
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cd src |
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tar xfvz ../deps/nucleominer-1.0.tar.gz |
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cd .. |
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.. code:: bash
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This creates a directory called that contains NucleoMiner 1.0 scripts (src/nucleominer-1.0/scripts).
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sudo make install |
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Generate .c2c Files |
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^^^^^^^^^^^^^^^^^^^ |
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.. code:: bash |
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mkdir dir_4_c2c |
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NMgxcomp ../data/saccharomyces_cerevisiae_BY_S288c_chromosomes.fasta\ |
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../data/saccharomyces_cerevisiae_rm11-1a_1_supercontigs.fasta\ |
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dir_4_c2c/BY_RM 2>dir_4_c2c/BY_RM.log |
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export PATH=$PATH:src/MUMmer3.23:src/nucleominer-1.0/scripts |
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export PERL5LIB=$PERL5LIB:src/nucleominer-1.0/scripts/ |
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NMgxcomp data/saccharomyces_cerevisiae_BY_S288c_chromosomes.fasta \ |
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data/saccharomyces_cerevisiae_rm11-1a_1_supercontigs.fasta \ |
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data/byxrm 2>NMgxcomp.log |
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After execution, the directory `dir_4_c2c` will hold the .c2c files. |
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After execution, the directory `data` will hold the .c2c files. |
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