153 |
153 |
nuc_width = 160, ##<< Nucleosome width.
|
154 |
154 |
only_f = FALSE, ##<< Filter only F reads.
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155 |
155 |
only_r = FALSE, ##<< Filter only R reads.
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156 |
|
filter_for_coverage = FALSE, ##<< Does it filter for plot coverage?
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157 |
|
USE_DPLYR = TRUE ##<< Use dplyr lib to filter reads.
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|
156 |
filter_for_coverage = FALSE ##<< Does it filter for plot coverage?
|
158 |
157 |
) {
|
159 |
|
n = names(inputs)
|
160 |
|
|
161 |
|
if (!USE_DPLYR) {
|
162 |
|
if (only_f) {
|
163 |
|
inputs_out = inputs[inputs[,1]==chr & inputs[,2] >= x_min - nuc_width & inputs[,3] == "F" & inputs[,2] <= x_max + nuc_width,]
|
164 |
|
} else if (only_r) {
|
165 |
|
inputs_out = inputs[inputs[,1]==chr & inputs[,2] >= x_min - nuc_width & inputs[,3] == "R" & inputs[,2] <= x_max + nuc_width,]
|
166 |
|
} else {
|
167 |
|
inputs_out = inputs[inputs[,1]==chr & inputs[,2] >= x_min - nuc_width & inputs[,2] <= x_max + nuc_width,]
|
168 |
|
}
|
169 |
|
} else {
|
170 |
|
names(inputs) = c("chr", "pos", "str", "lev")
|
171 |
|
if (only_f) {
|
172 |
|
inputs_out = filter(inputs, chr == chr, pos >= x_min - nuc_width, str == "F", pos <= x_max + nuc_width)
|
173 |
|
} else if (only_r) {
|
174 |
|
inputs_out = filter(inputs, chr == chr, pos >= x_min - nuc_width, str == "R" & pos <= x_max + nuc_width)
|
175 |
|
} else {
|
176 |
|
inputs_out = filter(inputs, chr == chr, pos >= x_min - nuc_width, pos <= x_max + nuc_width)
|
177 |
|
}
|
178 |
|
# if (!filter_for_coverage) {
|
179 |
|
# inputs$corrected_inputs_coords = inputs[,2] + nuc_width/2 * sign_from_strand(inputs[,3])
|
180 |
|
# inputs = filter(inputs, chr == chr, corrected_inputs_coords >= x_min, corrected_inputs_coords <= x_max)
|
181 |
|
# inputs$corrected_inputs_coords = NULL
|
182 |
|
# }
|
183 |
|
}
|
184 |
|
|
|
158 |
if (only_f) {
|
|
159 |
inputs = inputs[inputs[,1]==chr & inputs[,2] >= x_min - nuc_width & inputs[,3] == "F" & inputs[,2] <= x_max + nuc_width,]
|
|
160 |
} else if (only_r) {
|
|
161 |
inputs = inputs[inputs[,1]==chr & inputs[,2] >= x_min - nuc_width & inputs[,3] == "R" & inputs[,2] <= x_max + nuc_width,]
|
|
162 |
} else {
|
|
163 |
inputs = inputs[inputs[,1]==chr & inputs[,2] >= x_min - nuc_width & inputs[,2] <= x_max + nuc_width,]
|
|
164 |
}
|
185 |
165 |
if (!filter_for_coverage) {
|
186 |
|
corrected_inputs_coords = inputs_out[,2] + nuc_width/2 * sign_from_strand(inputs_out[,3])
|
187 |
|
inputs_out = inputs_out[inputs_out[,1]==chr & corrected_inputs_coords >= x_min & corrected_inputs_coords <= x_max,]
|
|
166 |
corrected_inputs_coords = inputs[,2] + nuc_width/2 * sign_from_strand(inputs[,3])
|
|
167 |
inputs = inputs[inputs[,1]==chr & corrected_inputs_coords >= x_min & corrected_inputs_coords <= x_max,]
|
188 |
168 |
}
|
189 |
|
|
190 |
|
names(inputs_out) = n
|
191 |
|
return(inputs_out)
|
|
169 |
return(inputs)
|
192 |
170 |
### Returns filtred inputs.
|
193 |
171 |
}
|
194 |
172 |
|
195 |
173 |
|
196 |
174 |
|
|
175 |
# filter_tf_inputs = function(# Filter TemplateFilter inputs
|
|
176 |
# ### This function filters TemplateFilter inputs according genome area observed properties. It takes into account reads that are at the frontier of this area and the strand of these reads.
|
|
177 |
# inputs, ##<< TF inputs to be filtered.
|
|
178 |
# chr, ##<< Chromosome observed, here chr is an integer.
|
|
179 |
# x_min, ##<< Coordinate of the first bp observed.
|
|
180 |
# x_max, ##<< Coordinate of the last bp observed.
|
|
181 |
# nuc_width = 160, ##<< Nucleosome width.
|
|
182 |
# only_f = FALSE, ##<< Filter only F reads.
|
|
183 |
# only_r = FALSE, ##<< Filter only R reads.
|
|
184 |
# filter_for_coverage = FALSE, ##<< Does it filter for plot coverage?
|
|
185 |
# USE_DPLYR = FALSE ##<< Use dplyr lib to filter reads.
|
|
186 |
# ) {
|
|
187 |
# n = names(inputs)
|
|
188 |
#
|
|
189 |
# if (!USE_DPLYR) {
|
|
190 |
# if (only_f) {
|
|
191 |
# inputs_out = inputs[inputs[,1]==chr & inputs[,2] >= x_min - nuc_width & inputs[,3] == "F" & inputs[,2] <= x_max + nuc_width,]
|
|
192 |
# } else if (only_r) {
|
|
193 |
# inputs_out = inputs[inputs[,1]==chr & inputs[,2] >= x_min - nuc_width & inputs[,3] == "R" & inputs[,2] <= x_max + nuc_width,]
|
|
194 |
# } else {
|
|
195 |
# inputs_out = inputs[inputs[,1]==chr & inputs[,2] >= x_min - nuc_width & inputs[,2] <= x_max + nuc_width,]
|
|
196 |
# }
|
|
197 |
# } else {
|
|
198 |
# names(inputs) = c("chr", "pos", "str", "lev")
|
|
199 |
# if (only_f) {
|
|
200 |
# inputs_out = filter(inputs, chr == chr, pos >= x_min - nuc_width, str == "F", pos <= x_max + nuc_width)
|
|
201 |
# } else if (only_r) {
|
|
202 |
# inputs_out = filter(inputs, chr == chr, pos >= x_min - nuc_width, str == "R" & pos <= x_max + nuc_width)
|
|
203 |
# } else {
|
|
204 |
# inputs_out = filter(inputs, chr == chr, pos >= x_min - nuc_width, pos <= x_max + nuc_width)
|
|
205 |
# }
|
|
206 |
# # if (!filter_for_coverage) {
|
|
207 |
# # inputs$corrected_inputs_coords = inputs[,2] + nuc_width/2 * sign_from_strand(inputs[,3])
|
|
208 |
# # inputs = filter(inputs, chr == chr, corrected_inputs_coords >= x_min, corrected_inputs_coords <= x_max)
|
|
209 |
# # inputs$corrected_inputs_coords = NULL
|
|
210 |
# # }
|
|
211 |
# }
|
|
212 |
#
|
|
213 |
# if (!filter_for_coverage) {
|
|
214 |
# corrected_inputs_coords = inputs_out[,2] + nuc_width/2 * sign_from_strand(inputs_out[,3])
|
|
215 |
# inputs_out = inputs_out[inputs_out[,1]==chr & corrected_inputs_coords >= x_min & corrected_inputs_coords <= x_max,]
|
|
216 |
# }
|
|
217 |
#
|
|
218 |
# names(inputs_out) = n
|
|
219 |
# return(inputs_out)
|
|
220 |
# ### Returns filtred inputs.
|
|
221 |
# }
|
|
222 |
|
|
223 |
|
|
224 |
|
197 |
225 |
get_comp_strand = function(
|
198 |
226 |
### Compute the complementatry strand.
|
199 |
227 |
strand ##<< The original strand.
|